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Duration of effect of HYB0432 on expression of TS protein in RKO cells. After a 6 h transfection with HYB0432–Eufectin complexes (190 nM), cells were harvested prior to transfection (lane 1) and at the following times: 0 (after transfection, lane 2), 6 (lane 3), 12 (lane 4), 24 (lane 5), 36 (lane 6), 48 (lane 7) and 72 h (lane 8). Cell extracts were processed for western blot analysis as described in Materials and Methods.
