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. 2001 Jan 15;29(2):415–422. doi: 10.1093/nar/29.2.415

Figure 4.

Figure 4

Effect of antisense treatment on expression of TS mRNA and TS protein. RKO cells were treated for 6 h in the absence (lane 1) or presence of oligo–Eufectin complexes (190 nM), grown for an additional 24 h, harvested and processed for northern (A) and western (B) blot analysis as described in Materials and Methods. Lanes 2–5 contain cell extracts treated with Eufectin alone (lane 2) or complexed with either HYB0432 (lane 3), a TS antisense 30mer PS-DNA oligo (lane 4) or TS sense 30mer RNA HYB0848 (lane 5).