Figure 5.

Effect of antisense treatment on the half-life of TS protein in RKO cells. Cells were incubated with Eufectin alone (squares) or Eufectin complexed with HYB0432 (190 nM) (circles) for 6 h, followed by a 2 h incubation with ³⁵S-labeled methionine-containing medium. After removal of the radiolabel, cells were harvested at the indicated times and processed as described in Materials and Methods. The amount of radiolabeled TS protein (treated with Eufectin alone) at 0 h equals 100%. Points represent the mean ± SD from three separate experiments.