Figure 7.

Effect of HYB0504 on expression of TS protein in TDX-resistant, RKO-TDX cells. Cells were treated for 12 h with increasing concentrations of HYB0504–Eufectin complexes: untreated (lane 2), 53 nM (lane 3), 105 nM (lane 4), 158 nM (lane 5), 315 nM (lane 6). Lanes 7 and 8 contain extracts from cells treated with 315 nM TSAS18-MM, a 5-base mismatch 18mer RNA and 210 nM TS18, a sense 18mer RNA, respectively. Lane 9 contains cell extracts treated with Eufectin alone (18 µg/ml). Lane 1 contains protein extract from the parent RKO cell line. After 24 h, cells were harvested and processed for western blot analysis as described in Materials and Methods.