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Effect of various nitrogen sources on PGase production from Bacillus sphaericus.
| Nitrogen source | Enzyme activity (μmol min−1 mL−1) | Relative activity (%) |
|---|---|---|
| (NH4)2SO4 | 4.0 ± 1.2 | 62.6 |
| (NH4)NO3 | 5.1 ± 0.3 | 80.2 |
| NH4Cl | 6.2 ± 0.6 | 95.9 |
| (NH4)2SO4·7H2O | 1.2 ± 0.9 | 19.5 |
| Ca(NO3)2 | 1.8 ± 0.3 | 28.3 |
| KNO3 | 2.4 ± 0.7 | 37.6 |
| NaNO3 | 2.6 ± 0.5 | 40.7 |
| (NH4)H2PO4 | 3.4 ± 0.2 | 52.9 |
| Urea | 1.7 ± 0.6 | 26.9 |
| Casein hydrolysate | 5.0 ± 0.9 | 78.6 |
| YE | 6.0 ± 0.6 | 93.3 |
| CH+YE (control) | 6.4 ± 0.8 | 100.0 |
Values are mean ± S.D. of 3 replicates.
