Figure 2.

Effect of temperature on the transfer efficiency of disulfide bond/tethered oligonucleotides from a glass surface to an acrylamide surface. Master-chips were prepared by spotting 50, 5 and 2 µM silanized Lac-thio solutions on the same glass slide. A separate master-chip was printed at room temperature, 75 and 95°C. After hybridizing with Lac-sen-Cy3, all slides were scanned at 25% laser power and 25% PMT gain. Signal intensities of the master-chips and their respective prints were summed and the percentage of oligonucleotides transferred to each print-chip was calculated accordingly.