Figure 3.

Effect of heating time on the transfer of covalently-immobilized oligonucleotides (via disulfide bonds) from a glass surface to an acrylamide surface. Master-chips were prepared by spotting 100 µM silanized Lac-thio solutions on the glass slides. Separate master-chips were printed at 95°C for 30 s and 1, 2, 3, 4, 5 and 6 min. After hybridization with the complimentary Lac-sen-Cy3, all slides were scanned at 25% laser power and 25% PMT gain. Signal intensities of the master-chips and their respective prints were summed and the percentage of oligonucleotides transferred to each print-chip was calculated accordingly.