Figure 3.
The hyperpolarization-activated current in GnRH neurons is carried by both Na+ and K+ ions. A, B, Representative voltage-clamp recordings showing current changes in response to reduction of extracellular Na+ (A, n = 6) or increase in extracellular K+ (B, n = 5). C, Top, Protocols used to establish reversal potential; bottom, representative voltage-clamp recording showing time of measurement of instantaneous current (○, left) and tail current (■, right). D, Calculation of reversal potential by linear fit of average voltages from C (n = 11). E, Effect of ionic manipulations on reversal potential (Vreversal). *p < 0.05.