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Analysis of RNA transcript integrity by IP RP HPLC. (A) RNA transcripts ranging in size from 749 to 2971 nt were analyzed individually by IP RP HPLC using gradient condition 2. The superimposed chromatograms of four individual transcripts are shown. (B) A crude transcription reaction product is shown. The full-length transcription product has a length of 5219 nt. Analysis was performed under elution condition 2.
