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Separation and purification of unlabeled and labeled β-actin RNA transcripts. DIG-labeled β-actin RNA transcript (1 µg) was analyzed by IP RP HPLC under elution condition 3. Unlabeled β-actin RNA elutes with a retention time of ∼4.7 min. DIG-labeled β-actin RNA elutes at the end of the gradient. Each peak was captured by manual collection.
