Figure 1.

Schematic representation of the method described in the article. Partial digestion and subsequent ligation with cassette DNA at both ends produces template DNA, which is then used for PCR amplification with different degenerated primer sets and a biotinylated cassette primer. Desired products as shown at the bottom of the figure are enriched via binding to streptavidin, reamplified in a second PCR, cloned and sequenced.