Table 1.
Values represent levels (pg/ml) of mediators in the culture supernatants of freshly-isolated (0-hour) and 24-hour, annexin V-negative (nonapoptotic, surviving) PMN following LPS stimulation, as assayed by multi-cytokine array and/or ELISA and as described in the Methods section
| Mediator | 0h |
24 h |
||||
|---|---|---|---|---|---|---|
| AD PMN | CBPMN | P | AD PMN | CBPMN | P | |
| Chemokines | ||||||
| ENA-78 | 8 ± 6 | 12 ± 6 | 0.43 | ND | ND | – |
| MCP-1 | 48 ± 3 | 51 ± 60 | 0.98 | ND | ND | – |
| RANTES | 86 ± 39 | 200 ± 94 | 0.07 | 14 ± 4 | 17 ± 4 | 0.31 |
| Cytokines | ||||||
| IL-1α | 10 ± 5 | 16 ± 2 | 0.07 | 10 ± 3 | 11 ± 5 | 0.69 |
| IL-1β | 28 ± 27 | 18 ± 9 | 0.98 | ND | ND | – |
| IL-6 | 14 ± 5 | 16 ± 1 | 0.62 | 9 ± 4 | 12 ± 19 | 0.64 |
| TNF-α | 43 ± 2 | 65 ± 13 | 0.02 | ND | ND | – |
| Anti-inflammatory mediators | ||||||
| IL-IRa | 6,565 ± 2,702* | 7,149 ± 992* | 0.69 | 949 ± 80 | 992 ± 235 | 0.74 |
| Angiogenic mediators | ||||||
| VEGF | 26 ± 4 | 52 ± 26 | 0.03 | ND | 7 ± 1 | – |
Data represent the means ± SD of data from 5 independent experiments using paired CB and AD PMN. ND = Levels below the assay limits of detection for that particular mediator.
*
p < 0.05, 0 h vs. 24-hour PMN.