Fig. 4.

STAT3 phosphorylation in the arcuate nucleus in response to i.p. leptin. Microphotographs of pSTAT3 immunohistochemistry from saline-treated CH rats (a), leptin-treated CH rats (b), saline-treated KD rats (c) and leptin-treated KD rats (d). Rats were treated with 2 mg/kg leptin and killed 30 min later. Images are representative matched sections, and each panel represents one animal. e The number of pSTAT3-positive cells is increased in KD rats as compared to CH rats, after saline and leptin treatment. * Statistically significant differences from the CH-saline group (p < 0.05).