Table 3.

Primer sequences for each gene are presented with the annealing temperature

Gene	Primer sequences	Temperature, °C
L32	Forward: 5'-CAG ACG CAC CAT CGA AGT TA-3'	60
	Reverse: 5'-AGC CAC AAA GGA CGT GTT TC-3'
SOCS3	Forward: 5'-TTC TTT ACC ACC GAC GGA AC-3'	60
	Reverse: 5'-TTT CCC ACA TCT TGT GAC CA-3'
Ob-Rb	Forward: 5'-TTC TTT ACC ACC GAC GGA AC-3'	60

RNA isolated from whole hypothalamus was used for cDNA synthesis. Quantitative PCR was used to compare hypothalamic gene expression compared to the L32 housekeeping gene.