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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biol Chem. 2010 Feb–Mar;391(2-3):259–270. doi: 10.1515/BC.2010.016

Figure 3. Activity measured after an immuno-precipitation assay performed on insect cell lysates.

Figure 3

Anti-FLAG antibody was used as a capturing antibody and protein G matrix was used as a solid support. Immunoprecipitated proteins were reacted with the cathepsin D/E FRET substrate for one hour in pH 3.5 buffer. Liberated product was measured on a fluorescent plate reader.