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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: Mol Microbiol. 2010 Apr 6;76(2):517–534. doi: 10.1111/j.1365-2958.2010.07119.x

Fig. 4.

Fig. 4

β-1,6-glucan dot blot assay. (A) Polysaccharide competition analysis. Alkali soluble cell wall material from H99 was spotted onto membrane. The primary anti-β-1,6-glucan antiserum was preincubated with the indicated purified polysaccharides for 30 min before probing the membrane. (B) Alkali-soluble cell wall β-1,6-glucan analysis of H99 and deletion strains. Cells were grown at 30°C to mid-log phase and alkali-soluble polysaccharides were isolated and spotted in 1:2 serial dilutions onto nitrocellulose membrane. Membranes were probed with polyclonal anti-β-1,6-glucan antiserum. (C) Alkali-insoluble, chitinase-released cell wall β-1,6-glucan analysis of H99 and deletion strains.