Figure 5.

Effect of inhibition of adherence to the α₄β₁or α₅β₁ integrins on WT and Nf1+/– mast cell haptotaxis. WT and Nf1+/– mast cells were preincubated with a neutralizing Ab to the α₄β₁ or α₅β₁ integrins, or an isotype control Ab, and haptotaxis assays were performed to rmKitL. Results represent the mean ± SEM of five independent experiments following a 3-hour stimulation to KitL. *P < 0.05 for WT or Nf1+/– mast cell migration in response to recombinant KitL in presence or absence of the α₄β₁-neutralizing Ab or the isotype Ab control using a Student’s paired t test.