Figure 6.

F4/80⁺ cells in adipose tissue are bone marrow–derived. Adipose tissue was collected and SVCs were isolated 6 weeks after lethal irradiation and bone marrow transplantation. The SVCs were incubated with APC-conjugated anti-F4/80 (F4/80-APC and either PE-conjugated anti-CD45.1 (CD45.1-PE) or PE-conjugated anti-CD45.2 (CD45.2-PE). Eighty-five percent of F4/80⁺ cells expressed the donor antigen, CD45.1 (right upper quadrant in a). Only 14% of the F4/80⁺ cells also expressed the recipient antigen, CD45.2 (right upper quadrant in b).