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. 2003 Dec 15;112(12):1821–1830. doi: 10.1172/JCI19451

Figure 1.

Figure 1

(a) The transcriptional regulation of inflammation genes in the WAT of mice with genetic or diet-induced obesity/diabetes by quantitative RT-PCR (TaqMan). For comparison, the expression level of these genes in lean mice was arbitrarily set at 1; error bars represent ± SE. LF, low fat (10% fat); HF, high fat (60% fat). ob/ob and db/db mice and appropriate controls (n = 5 per group) were obtained from The Jackson Laboratory, fed a standard chow diet (Farmer’s Exchange), and sacrificed at 15 weeks of age. DIO mice (C57BL/6J; The Jackson Laboratory) were obtained at 4 weeks of age and placed on the designated diet of 60% kcal from fat (Research Diets Inc.) for 16 weeks (n = 10 per group). y axes show arbitrary units representing relative expression levels of mRNAs. (b) Confirmation of inflammation-gene regulation by Northern blot analysis. An independent set of animals from the same experiment was used.