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. 2003 Dec 15;112(12):1831–1842. doi: 10.1172/JCI19774

Figure 5.

Figure 5

Effects of hyperglycemia and obesity on β cell dysfunction in WT and UCP2-deficient islets, with or without overexpression of MnSOD and GPX1. Pancreatic islets were isolated from WT (a) or UCP2 KO mice (b) and subjected to chronic incubations at low and high glucose. In a separate experiment, islets were isolated from ob/ob mice (c). Adenovirus-driven overexpression of MnSOD and GPX1 was used where indicated. Islets were incubated for a total of 72 hours and washed, and then insulin-secretion studies were performed using three different concentrations of glucose (5.5, 12.5, and 25 mM). Insulin- and DNA-content data are reported for reference in Table 1. Results are means ± SEM of three independent experiments (a and b) or five repeats of one representative experiment (c). *P < 0.05, higher glucose vs. lowest glucose condition in each group; #P < 0.05, basal secretion (chronic hyperglycemia) vs. basal secretion (5.5 mM glucose).