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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nat Neurosci. 2010 Jul 25;13(8):951–957. doi: 10.1038/nn.2601

Figure 2.

Figure 2

Effect of the ABHD6 inhibitor WWL70 and the MAGL inhibitor JZL184 on [3H]–2-AG hydrolysis in cell homogenates. [3H]–2-AG hydrolysis in homogenates prepared from (a,b) COS-7 cells transfected with mouse ABHD6 (5 μg of protein per reaction) or with mouse MAGL (0.25 μg of protein per reaction); (c) BV-2 cells (10 μg of protein per reaction); and (d) mouse neurons in primary culture (1 μg of protein per reaction). For the combination of the two inhibitors in d, WWL70 was used at 10 μM and JZL184 at 1 μM. Data are mean ± s.e.m. of 3 to 4 independent experiments. Each experiment was performed in triplicate with homogenates or cells from independent transfections and cell preparations. Transfection of COS-7 cells with ABHD6 increased [3H]–2-AG hydrolysis by about twofold compared to COS-7 cells transfected with empty vector, whereas transfection of COS-7 cells with MAGL increased [3H]–2-AG hydrolysis by 17-fold compared to COS-7 cells transfected with empty vector (data not shown).