Table 1. Cytosine DNA methylation in queens and workers in CG, CHG, and CHH genomic contexts (H = A, T, or C).
Total | Methylated in Queens | Methylated in Workers | Methylated in Both Castes | |
CG | 10,030,209 | 69,064 | 68,222 | 54,312 |
CHG | 8,673,113 | 14 | 130 | 0 |
CHH | 45,072,611 | 561 | 3,019a | 0 |
The thresholds used for methylation calls are detailed in the Methylation Assessment section.
Nearly all of the 3,019 CHH that were inferred to be methylated in worker brains on the basis of Solexa reads were found to be not methylated by an additional sequencing of selected amplicons using the 454 technology.