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. 2010 Nov 2;5(11):e15410. doi: 10.1371/journal.pone.0015410

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) AgApoLp-III mRNA expression in midguts of female mosquitoes fed on a healthy (control) or a P. berghei-infected mouse. Samples were collected immediately (0 hour) or 24 hours post feeding. Ribosomal S7 protein was amplified as reference. (B) Same as (A) but in carcass samples collected from the same mosquitoes. (C) AgApoLp-III expression in control and infected midguts kept at a temperature permissive for ookinete development (20°C) or at a non-permissive temperature (28°C). (D) Time course of AgApoLp-III induction in female mosquitoes in which gut microbiota have been removed with oral antibiotics. (E) Effect of actinomycin D-induced apoptosis on mRNA expression of dual oxidase (Duox) and AgApoLp-III (Apo-III).