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. 2010 Nov 2;5(11):e15410. doi: 10.1371/journal.pone.0015410

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Western blot analysis of hemolymph from adult females probed with pre-immune or polyclonal anti-AgApoLp-III antiserum. A single band of expected molecular weight (19 kDa) for the mature protein was detected and is indicated by the arrow. (B–G) Subcellular localization of AgApoLp-III (green) in control (B and F) and infected (C, D, E, and G) midguts. The ookinete surface was stained using anti-Pb28 monoclonal antibodies (red) and nuclei with DAPI (blue). Phase contrast images of the midgut surface in control (F) and infected (G) midguts are shown in the right panels.