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List of Primers
| Primer | Sequence | Size (bp) | TA (°C) | TM (°C) | Optimal Cycles |
|---|---|---|---|---|---|
| GAPDH | 5′-TCACCATCTTCCAGGAGCGA-3′ (forward) 5′-AGTGATGGCATGGACTGTGG-3′ (reverse) |
326 | 65 | 62 | 23 |
| α-SMA | 5′-GCTCACGGAGGCACCCCTGAA-3′ (forward) 5′-CTGATAGGACATTGTTGACAT-3′ (reverse) |
591 | 60 | 58 | 35 |
TA = annealing temperature; TM = melting temperature; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; α-SMA = alpha-smooth muscle actin
