TABLE 3.
Protein | Primary Antibody |
Secondary Antibody |
||
---|---|---|---|---|
Antibody | Dilution | Antibody | Dilution | |
α-SMA | Anti α-SMA monoclonal antibody | 5 μg/mL | Sheep anti-mouse IgG horseradish conjugate | 1:100 |
Laminin | Rabbit anti-laminin | 1:25 | Goat anti-rabbit IgG antibody conjugated with alexa fluor 488 | 1:1000 |
α-SMA = alpha-smooth muscle actin