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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Cell Res. 2010 Aug 10;20(11):1263–1275. doi: 10.1038/cr.2010.115

Figure 2. Tyrosine kinase activity of various EphA3 mutants.

Figure 2

(A) Effects of the tyrosine to phenylalanine mutations on EphA3 kinase activity. EphA3 was immunoprecipitated from transfected 293A cells and incubated with 10 μg of acid denatured Enolase. The reaction was stopped by adding SDS sample loading buffer and boiling. The reaction products were analyzed by immunoblotting with anti-phosphotyrosine antibody (upper panel) and anti-EphA3 antibody (lower panel), respectively. (B) Tyrosine kinase activity of the double and triple EphA3 mutants. (C) Receptor autophosphorylation levels in the double and triple EphA3 mutants.