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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Cell Res. 2010 Aug 10;20(11):1263–1275. doi: 10.1038/cr.2010.115

Figure 3. Phosphorylation of both Y602 and Y779 contributes to the inhibition of cell migration by EphA3.

Figure 3

(A) Inhibition of cell migration by EphA3 is kinase-dependent. 293A cells were transfected with wild type EphA3, kinase-dead mutant K653R, or pCMV vector only, and assessed for effects on cell migration using the wound-healing assay. Images were taken at 0 and 24 hours after wounding. (B) Quantification of effects of EphA3 tyrosine mutants on cell migration in the wound-healing assay. (C) Quantification of effects of EphA3 tyrosine mutations on cell migration in the Transwell assay. In both assays, cell counts were normalized to that of the vector controls. Bars represent the means of six independent experiments. Error bars indicate standard deviation. * indicates α < 0.05 vs wild type (WT) (one-way ANOVA followed with Bonferroni test for selected pairs).