. Author manuscript; available in PMC: 2011 May 1.

Published in final edited form as: Cell Res. 2010 Aug 10;20(11):1263–1275. doi: 10.1038/cr.2010.115

Table 1. Primers used in site-directed mutagenesis.

Mutations	Mutagenic Primers (Mutated nucleotides are underlined)

Y569F	5′-GGAGGTTTTGTGGCTTTCACAAGTCAAAACACAG-3′
Y596F	5′-CTCAGGACCTTTGTTGATCCAC-3′
Y602F	5′-GATCCACATACATTTGAAGACCCTACTC-3′
Y659F	5′-CCCTGAAAGTAGGGTTTACCGAGAAACAGAGG-3′
Y701F	5′-GTCACGGAATTCATGGAGAATGGC-3′
Y736F	5′-GCATCAGGTATGAAATTCCTCTCAGATATGGG-3′
Y742F	5′-GATATGGGCTTCGTCCACCGG-3′
Y779F	5′-GAAGCTGCTTTCACAACCAGG-3′
Y798F	5′-GCAATTGCCTTCCGCAAGTTC-3′
Y810F	5′-CCAGCGATGTATGGAGTTTCGGGATTGTTC-3′
Y820F	5′-GGGAAGTGATGTCTTTCGGAGAAAGGCC-3′
Y825F	5′-GAAAGGCCATTCTGGGAGATG-3′
Y841F	5′-GGATGAGCGGTTTCGCCTGCCACC-3′
Y854F	5′-CCAGCTGCCTTGTTTCAGTTGATGTTGG-3′
Y937F	5′-GGCGTCGAATTCAGCTCCTGTG-3′

Y596E	5′-GGTCTCAGGACCGAGGTTGATCCACATAC-3′
Y602E	5′-GATCCACATACAGAGGAAGACCCTACTC-3′
Y779E	5′-AGAAGCTGCTGAGACAACCAGGGG-3′

K653R	5′-GGTGGCCATAAGGACCCTGAAAG-3′