Figure 1. SOD mRNA levels are altered with M. abscessus infection.
Differentiated THP-1 cells were uninfected (control), infected with M. abscessus for the indicated times. Cells were harvested at the times indicated and mRNA was isolated. A. Real Time RT-PCR analysis for MnSOD mRNA levels. MnSOD mRNA was significantly elevated with infection. B. Real Time RT-PCR analysis for Cu/ZnSOD mRNA levels. Cu/ZnSOD mRNA levels were unchanged early during infection; however, at 48 hours post-infection Cu/ZnSOD levels declined (not significant). All data represent the mean ± SEM, n=4. The asterisk (*) denotes significant change from the respective control (p< 0.05).
