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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2010 Aug 31;49(11):1666–1673. doi: 10.1016/j.freeradbiomed.2010.08.026

Figure 3. SOD activity is altered with M. abscessus infection.

Figure 3

Differentiated THP-1 cells were uninfected (control), infected with M. abscessus (M. abs) or infected in the presence of MnTE-2-PyP (M. abs + MnTE-2-PyP) for 24, 48 or 72 hours. A. A representative nitroblue tetrazolium gel. B. Densitometric analysis of MnSOD activity. MnSOD activity was significantly enhanced in infected cells as compared to uninfected cells at 24 hours post-infection. At 48 and 72 hours post-infection MnSOD activity was enhanced in infected cells as compared to control (not significant). C. Densitometric analysis of Cu/ZnSOD activity. Cu/ZnSOD activity was significantly decreased at 72 hours post-infection in M. abscessus infected cells as compared to uninfected cells. All data was made relative to the 24 hour uninfected time point for the respective SOD. For controls, media, FBS and M. abscessus were analyzed for SOD activity. All data represent the mean ± SEM, n=4. The asterisk (*) denotes significant change from the respective control (p< 0.05).