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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cancer Res. 2010 Oct 12;70(21):8948–8958. doi: 10.1158/0008-5472.CAN-10-1936

Figure 1.

Figure 1

uPAR over-expression in MDA-MB-468 cells induces CSC-like properties. A, Flow cytometry to detect uPAR in 468/uPAR (heavy curve) and 468/EV (light curve) cells. B, CD24 and CD44 were determined in 468/uPAR and 468/EV cells. Isotype-matched IgG controls are presented in Supplemental Figure 1A. C, Total β1/CD29, activated β1, and α6/CD49f were determined in 468/uPAR (heavy curve) and 468/EV (light curve) cells. The isotype-matched IgG is shown with a dashed line. D, α6 integrin and tubulin in affinity precipitates and whole-cell extracts from 468/EV and 468/uPAR cells were determined. E, Representative mammosphere formed by 468/uPAR cells (Bar, 50 µm). F, 468/uPAR and 468/EV cells were introduced into suspension culture at the indicated densities. Mammospheres were counted. The bar graph shows one of three independent experiments, which generated similar results (mean ± SEM). G, Single-cell cultures were maintained for 7–10 days before determining the frequency of mammosphere formation (n=4, mean ± SEM).