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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cancer Res. 2010 Oct 26;70(21):8329–8338. doi: 10.1158/0008-5472.CAN-10-2179

Figure 3. RANKL expression is elevated in tumors formed by HPSE-high cells.

Figure 3

A. Human RANKL staining within human bones from SCID-hu mice bearing tumors formed by HPSE-low or HPSE-high cells. The red asterisks denote bone and inserts are controls lacking addition of primary antibody (original magnification 400x). B. Primary myeloma tumors formed by HPSE-low or HPSE-high cells growing subcutaneously in SCID mice were extracted with detergent and western blotting was performed for detection of RANKL, OPG, and β-actin. Each lane of the blot represents tumor from a different animal. C. The bands from western blots were quantified relative to β-actin using ImageJ software. The bars represent means ± SD.