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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Arthritis Rheum. 2010 Nov;62(11):3322–3333. doi: 10.1002/art.27659

Figure 6.

Figure 6

A. SMC were stimulated with graded concentrations of FcγRIIa crosslinker with or without C5a 10nM × 4h. C5a contributed to SMC degranulation but did not alter responsiveness to FcγRIIa. B. SMC were stimulated × 4h with C5a 10nM, FcγRIIa crosslinker (5µL), or both; ** p<0.01. Data in A and B representative of 5 independent experiments using SMC from 2 donors. C. FcγRII−/− BMMC in the bottom of a transwell system were stimulated via C5a 50nM, 2.4G2 10ug/ml or both, and migration of C5aR−/− neutrophils from the upper chamber quantitated at 4h by flow cytometry. C5a did not result in enhanced migration. Representative of 2 experiments. D. Presence of extravasated NIMP-R14+ neutrophils was assessed 24h after i.p. instillation of 200uL K/BxN serum; n=5 mice (9 ankles)/group.