Figure 5.

Co-immunoprecipitation of GFP-Bax and GFP-Bcl-xL/Bax H5 and the mitofusins. (a) HeLa cells were transfected with FLAG-Mfn2 and GFP, GFP-Bax, GFP-Bax A117P (mutation within H5), GFP-Bcl-xL/Bax H5 or GFP-Bax/Bcl-xL H5 (the reverse H5 chimera) and immunoprecipitated with FLAG beads and blotted for FLAG and GFP. (b) WT HCT116 and EndoB1^−/−EndoB2^−/− HCT116 cells transfected with FLAG-Mfn2 and GFP-Bax or GFP-Bcl-xL/Bax H5 were immunoprecipitated with FLAG beads and blotted for FLAG, GFP, EndoB1 and EndoB2. (c) Drp1 RNAi or control GFP RNAi HeLa cells³¹ were transfected with FLAG-Mfn2 and GFP-Bax and immunoprecipated and blotted as in (a). Drp1 protein level after RNA interference treatment was analyzed using anti-Drp1 antibody. Asterisks represent nonspecific bands. These data are representative of at least two independent experiments