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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: FEMS Microbiol Lett. 2010 Aug 27;312(1):24–32. doi: 10.1111/j.1574-6968.2010.02093.x

Fig. 3.

Fig. 3

(a) Proteolytic activity of ECF sigma factor mutants. P. gingivalis strains were grown to stationary phase in BHI media supplemented with hemin and vitamin K. Activities against Rgp or Kgp were tested in whole cell culture. The gingipain activities were normalized to W83 being 100% and the mutants reported as a percentage thereof. Asterisks indicate results significantly different from those of W83 (P<0.005). Error bars represent the standard deviation. A, Rgp activity. B, Kgp activity. C, Rgp activity of FLL350 complemented with PG0162. D, Kgp activity of FLL350 complemented with PG0162. (b) RT-PCR analysis of gingipain encoding genes in W83, FLL350 and FLL354.