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Centrin in human sperm detected by Western blot analysis. Lanes 1 and 2 - Centrins in normozoospermic and OA males. Lane M - Broad range rainbow protein molecular weight marker (12,000 – 2,25,000, Amersham Biosciences now GE Healthcare Lifesciences). Lane 3 - Negative control (monoclonal antibody was substituted with 0.01 M PBS)
