Figure 10.

Doxycycline-induced overexpression of macrophage migration inhibitory factor (MIF) (on Days 1–14) in the lungs of Clara-cell secretory protein (CCSP)-rtTA/tet(O)-MIF double-transgenic mice results in increased proliferation and protection from apoptosis in subsequently introduced orthotopic Lewis lung carcinoma tumors (Days 14–28). (A) shows the number of apoptotic cells per high-power field (cleaved caspase 3) in tumors from double- (+/+) or single-transgenic (+/−) mice treated with or without doxycycline (P = 0.006 by analysis of variance [ANOVA]). (B) shows the proliferative index (% of Ki67-positive cells) in tumors from double- (+/+) or single-transgenic (+/−) mice treated with or without doxycycline (P = 0.002 by ANOVA). Control mice include double-transgenic mice receiving no doxycycline and single-transgenic mice with or without doxycycline. +/+ designates mice bearing both the CCSP-rtTA and tet(O)MIF alleles, respectively. +/− designates single-transgenic littermate mice. Each bar represents the analysis of tumors from the lungs of at least five mice per group.