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. 2010 Jun 17;182(8):1073–1079. doi: 10.1164/rccm.201003-0334OC

Figure 4.

Figure 4.

Frequency and cytokine expression profile of bacillus Calmette-Guérin (BCG)–specific CD8 T cells. (A) Frequencies of total, IL-2, IFN-γ, tumor necrosis factor (TNF)–α, and IL-17 cytokine-expressing CD8 T cells, as detected by an intracellular cytokine assay after incubation of whole blood with BCG. Total cytokine frequencies incorporate all cytokine-positive CD8 T cells One participant had a very high total and IFN-γ CD4 T cell response (6.198 and 6.157%, respectively), and these values are shown individually on the graph. (B) Frequencies of distinct subsets of specific CD8 T cells, based on combinations of cytokine expression. After selecting CD8 T cells, boolean gating was used to generate 15 distinct cytokine-expressing subsets. One participant had a very high IL-2 CD4 T cell response (0.199%), and this value is shown individually on the graph. The Kruskal-Wallis test was used to assess differences between the groups of infants. None of the parameters assessed was different between groups (all: P > 0.05).