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. Author manuscript; available in PMC: 2011 Dec 5.
Published in final edited form as: Virology. 2010 Dec 5;408(1):119–127. doi: 10.1016/j.virol.2010.09.005

Fig. 1. HIV-1 enters podocytes by a dynamin-dependent endocytosis.

Fig. 1

(A) Differentiated AB 8/13 podocytes were incubated for 90 min with fluorescently labeled HIV-1 AD8, washed and incubated for 30 min with LysoTracker Red (LT, 50 nM) or Transferrin-Alexa Fluor 594 (TF, 5 μg/ml). Separate and merged confocal microscopy images are shown. (B) Podocytes were pretreated for 1 h with 200 μM dynasore in 0.1% DMSO (+Dynasore) or with 0.1% DMSO alone (−Dynasore) and infected with AD8 as described in (A). Confocal images of AD8 entry and Transferrin-Alexa Fluor 594 uptake in the absence or presence of dynasore are shown. (C, D) Colocalization of HIV-1 particles (multiplicity of infection 100-fold lower than in Fig. 1A and B) with LysoTracker Red (LT) and Transferrin-Alexa Fluor 594 (TF) in podocytes (C) and TZM-bl cells susceptible to HIV-1 infection (D).