Fig. 4.

Fermentation kinetics by immobilized mixed culture of AFF01/pLOI297 and CT1101/pLOI297. The experiments were performed in capped shake flasks containing 100 ml of semi-defined medium supplied with sugar mixtures and with 35 ml beads containing cell concentration of 10.2 g-CDW/l-gel. Optimal cell fraction f_opt was determined from the kinetic model (see Equ. (14)). (A) Relation of consumed xylose with an initial concentration of 60 g/l and consumed glucose with an initial concentration of 20 g/l at the fraction of CT1101/pLOI297 cells used in the immobilization: f = 0.27 (), f = 0.34 (), f = 0 (). (B) Relation of consumed sugars in the mixture: glucose-xylose at cell fraction f_opt = 0.41 (), glucose-arabinose at cell fraction f_opt = 0.47 (), and arabinose-xylose at cell fraction f_opt = 0.58 (). (C) Immobilized mixed culture at the optimal cell fraction in a sugar mixture of glucose () and xylose () converted into ethanol (). (D) Immobilized mixed cultures at the optimal cell fraction in a sugar mixture of glucose and arabinose (). (E) Immobilized mixed cultures at the optimal cell fraction in a sugar mixture of xylose and arabinose.