Table 1. Εσχηɛριχηια χολι γλνΗπ2 and S.meliloti nifH promoter sequences.
Promoter |
Plasmid |
Sequence |
Reference |
–28 –13 +1 | |||
E.coli glnHp2 | pFC50 | ACTGGCACGATTTTTTCATATATGTGAATGT | 34 |
pFC50-m12 | ACTGGCACGATTTTTGCATATATGTGAATGT | 34 | |
pFC50-m33 | ACTGGCACGATTTTTCCATATATGTGAATGT | 34 | |
pFC50-m11 | ACTGGCACGATTTTTACATATATGTGAATGT | 34 | |
–29 –14 +1 | |||
S.meliloti nifH | pMKC28 | GCTGGCACGACTTTTGCACGATCAGCCCTGGG | 36 |
–28 –13 +1 | |||
K.pneumoniae glnAp2 | – | GTTGGCACAGATTTCGCTTTATATTTTTTAA |
The E.coli glnHp2 (shown from –28 to +3) and S.meliloti nifH (shown from –29 to +3) promoter sequences used for the in vitro transcription assays. The –13GC (for the glnHp2 promoters) and –14GC (for the nifH promoter) promoter elements are underlined and the consensus G is shown in bold. The K.pneumoniae glnAp2 promoter sequence (from –28 to +3) is also given for comparison.