Table 2. The S.meliloti nifH and E.coli glnHp2 DNA fragments used for the gel mobility shift assays.

The S.meliloti nifH and E.coli glnHp2-13T→G (–12) heteroduplex promoter DNA fragments (from –60 to +28) used for the gel mobility shift assays. Shown are sequences from –26 to +3, where the σ⁵⁴ consensus promoter sequences are in bold and mutant sequences introduced in the top strand to create the mismatch are boxed in black (16,27).