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Preparation of GST–YB-1 mutants and double- and single-stranded DNA binding by YB-1. (A) Diagram of YB-1 protein and its derivatives. The CSD and the basic (b) and acidic (a) amino acid clusters are indicated for the wild-type YB-1 protein. (B) Approximately 100 ng GST fusion protein were incubated with the double-stranded target oligonucleotide (25mer) including the MDR1 Y box (18). (C) Approximately 100 ng fusion proteins were incubated with the oligonucleotide (32mer).
