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. Author manuscript; available in PMC: 2010 Nov 3.
Published in final edited form as: Reproduction. 2009 Nov 9;139(2):395–407. doi: 10.1530/REP-09-0182

Figure 3.

Figure 3

The asssessment of Caspase-3 dependent apoptosis over sheep gestation. A, Light field-microscopy revealed intense brown DAB staining for cleaved Caspase-3 in the germ cells of Stage 1 ovaries. Decreased cleaved Caspase-3 expression was noted in the germ cells of Stage 2 (B) and Stage 3 (C) ovaries. Black arrows are pointing at oocytes showing positive brown DAB staining. D, No DAB staining was observed in the negative control section. All the scale bars represent 100 μm. E, To quantify the results, the total number of activated Caspase-3 positive cells were counted over 8-12 fields for each ovary. This number was divided by the total area of 8-12 fields (2000-3000 μm2) to give the number of cells per μm2 for each ovary. For this experiment three Stage 1; eight Stage 2 and four Stage 3 ovaries were examined for cleaved Caspase-3 staining. Values are the mean for each group ± SEM. Expression of activated Caspase-3 did not change significantly across gestation (P>0.05, Kruskal-Wallis).