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The amount of TNF-α protein secreted after transfection of TOV-122D cells with pH19 -TNF plas-mid determined by ELISA. Medium from TOV-122D transfected cell was collected after 48h. TNF-α protein level was analyzed in the supernatant using a TNF-specific ELISA. The amount of TNF-α protein was normalized to a standard curve with increasing concentrations of TNF-α.
