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. 2010 Sep 21;3(4):270–282.

Figure 3.

Figure 3

The reduction of luciferase activity in SK-OV3 cell line due to co-transfection with the pH19-DTA and pH19-TNF vectors, compared with transfection of the pH19-TNF-IRES-DTA vector. The killing potential of the pH19-DTA and pH19-TNF vectors or pH19-TNF-IRES-DTA was measured as a reduction of Luc-SV40 activity. Cells were transfected with Luc-SV40 (2 μg/well) and 0.0125 μg/well of each of the following vectors:pH19-DTA, pH19-TNF, orpH19-TNF -IRES-DTA, co-transfected with pH19-DTA and pH19-TNF 0.0125 μg/well of each plasmid). Cells were also transfected with LucSV40 alone. Transfection experiments were stopped after 48 hours and reporter gene activity was assessed.