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. 2010 Sep 21;3(4):270–282.

Figure 4.

Figure 4

The reduction of luciferase activity in SK-OV3 and ES-2 cell lines transfected with LucSV40 due to transfection with pH19-DTA, pH19-TNF-IRES-DTA or pH19-TNFrev-IRES-DTA vectors. The killing potential of the pH19-DTA and pH19-TNF-IRES-DTA or pH19-TNFrev-IRES-DTA was measured as a reduction of luciferase activity in SK-OV3 cell line (A) and in ES-2 cell line (B). Cells were transfected with 2 μg/well of Luc-SV40 and the indicated concentration of pH19-DTA, pH19-TNF-IRES-DTA, pH19-TNFrevDTA respectively. Cell lines were also transfected with Luc-SV40 alone. Transfection experiments were stopped after 48 hours and reporter gene activity was assessed. The luciferase activity in the co-transfected cells was compared to the luciferase activity of the cells transfected with the Luc-SV40 plas-mid alone.