Figure 5.

The effect of intratumoral injection of the pH19-TNF-IRES-DTA, pH19 -TNFα, pH19-DTA or pH19-Luc plasmid on the growth of subcutaneous ovarian tumors in nude mice. After tumors developed, mice were divided into 4 groups of 6 mice. Mice received 4 injections of 25 μg of pH19-TNF-IRES-DTA, pH19 -TNFα, pH19-DTA or pH19-Luc plasmids complexed with PEI within two day intervals. One day after the last treatment animals were sacrificed. The tumor dimensions were measured in vivo prior to the first treatment and immediately before sacrifice. The mean fold increase of the final volume compared to the initial volume in the treated tumors was calculated. A and B represents tumors developed by ES-2 or by SK-OV3 cells respectively.