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. 2010 Sep 27;3(4):303–307.

Figure 3.

Figure 3

Effect of fatty acids on TLR4-mediated NF-κB activation. HEK-Blue cells were grew to 90% con-fluency and treated with 200 μl 100 μM fatty palmitic acid, stearic acid, oleic acid or arachidonic acid solution respectively, or 1% BSA in PBS as a control for 24 h. 100 μl of the culture supernatant was mixed with 100 μl HEK-Blue detection medium and incubated at 37°C for 2 h. Absorption at 650 nm was measured to quantify the blue color. Saturated fatty acids promote TLR4-mediated NF-κB activation while unsaturated fatty acids suppress TLR4-mediated NF-κB activation. Data are expressed as means ± SD of triplicate determinations. **P < 0.01 versus cells with no fatty acid solution or BSA treatment.